prozyme glycan standards

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January 8, 2018

prozyme glycan standards

Blackrock, Ireland: National Institute for Bioprocessing Research and Training (NIBRT); c2012. Founded in 1990, Hayward-Calif. based ProZyme is an industry leading developer and manufacturer of glycan … Given the stoichiometry of the Schiff base and activated carbamate labelling reactions, each glycan can get only one fluorophore and direct relative quantitation by HPLC-FLD is possible. Agilent unlabeled N-glycan (formerly ProZyme) standards are used as qualitative standards for LC/FLD, LC/MS, or CE/LIF. 15 Reductive Symbol and Text Nomenclature for Representation of Glycan Structure. corresponding ProZyme glycan standards (product codes GKC-124300, GKC-224300 and GKC-335300, respectively) with the GKK-404 Signal™ 2-AB Labeling Kit. Founded in 1990, ProZyme is an industry-leading developer and manufacturer of glycan reagents, kits and standards, which are required for efficient sample prep in the analysis of free glycans. Glycobiology, which is the study of the structure, function and biology of carbohydrates, also called glycans, is a fast-growing and important field of study in life sciences. Each batch of ACQUITY UPLC BEH Glycan column packing material is physically, chemically, and chromatography tested using a well characterized 2AB-labeled glycan standard. ProZyme salary trends based on salaries posted anonymously by ProZyme employees. Push your Glycoprotein analysis to new levels of performance. N-glycan (asialo, agalacto biantennary complex) labeled with 2-aminobenzoic acid (2-AA, also known as anthranilic acid), for use as a qualitative standard in applications such as liquid chromatography (LC). The GlycoClean™ S Cartridges were purchased from ProZyme® (Hayward, … determining the percentage of each glycan species. Qualitative standard for glycan identification. Events & Trade Shows |  For glycan masses, see the product pages and our downloadable Excel file listing masses of adduct ions of unlabeled and labeled glycans for mass spectrometry work. Agilent Biocolumns - Critical Quality Attributes - Application Compendium. Founded in 1990, ProZyme is an industry-leading developer and manufacturer of glycan reagents, kits and standards, which are required for efficient sample prep in the analysis of free glycans. GlycoWorkbench: a tool for the computer-assisted annotation of mass spectra of glycans. The positive mode tab shows glycans labeled by 2-AB, InstantAB, 2-AA, Procainamide and InstantPC. Qualitative standard for glycan identification. is correlated to glycan structures that have been previously determined. AdvanceBio G0F / FA2 N-glycan standard (formerly ProZyme). Web Site Use Guidelines |  Glycan Release • The Protein Express 200 assay was run on the LabChip platform as a control to test for the level of deglycosylation. The Glycan Labeling Kit was purchased from QA-Bio, Inc. (Burlington, ON). Alternative glycan names: F … Certificates of Analysis |  N-glycan (asialo-, agalacto-, biantennary complex N-glycan, core-substituted with fucose). Alternatively, if a mixture of known standards is available, these standards can be analyzed under the same chromatographic conditions as the samples. GlycoBase 3.2.4. Choose labeled or unlabeled standards. AdvanceBio 2-AB G1 / A2G1 N-glycan standard (formerly ProZyme). Careers |  Products :  HAYWARD, Calif., Nov. 14, 2016 /PRNewswire-iReach/ -- ProZyme, Inc., a leading manufacturer of reagents and tools for glycobiology, today announced the launch of the Gly-Q Glycan Analysis System, an integrated platform for high-throughput, user-friendly glycoanalysis. The positive mode tab shows glycans labeled by 2-AB, InstantAB, 2-AA, Procainamide and InstantPC. News |  N-glycan (asialo-, mono-galactosylated biantennary) labeled with 2-aminobenzamide (2-AB), for use as a qualitative standard in applications such as liquid chromatography (LC). Nomenclature Committee, Consortium for Functional Glycomics; [2012; cited 2015 Oct 6]. Alternative glycan names: NGA2. Supervised production of ProZyme's line of glycan standards. [Internet]. The Glyko® 2-AB Human IgG N-Linked Glycan Library was purchased from ProZyme® (Hayward, CA), and the 2-AB labeled IgG N-Glycan Library was purchased from Ludger Ltd (Oxfordshire, U.K.). Part Number: GKR-002300. Founded in 1990, ProZyme is an industry leading developer and manufacturer of glycan reagents, kits and standards, which are required for efficient sample prep in the analysis of free glycans. About Prozyme :  An assortment of standards and libraries are available. Glyko Standards. The negative mode tab shows glycans labeled by 2-AB, InstantAB, 2-AA, Procainamide and APTS. SANTA CLARA, Calif.--(BUSINESS WIRE)-- Agilent Technologies Inc. (NYSE:A) today announced that it has completed the acquisition of ProZyme, Inc., a leading provider of glycan reagents, kits and standards.This acquisition expands Agilent’s portfolio of biopharma consumables. Glycan Performance Test Standard . Add to Favorites. At the front end of the Gly-Q system, enzymatically-released and fluorescently-labeled glycans will be prepared using ProZyme chemistry prior to glycan separation using the automated CGE instrument. For more details on our glycan standards, or to request a new glycan standard product, please contact us. For glycan masses, see the product pages and our downloadable Excel file listing masses of adduct ions of unlabeled and labeled glycans for mass spectrometry work. The Glycan Performance Test Standard line is intended to be used as a benchmarking standard for ACQUITY UPLC BEH Glycan, 1.7 µm Columns. ProZyme core fucosylated n glycans 2 ab labeled n glycan standards Core Fucosylated N Glycans 2 Ab Labeled N Glycan Standards, supplied by ProZyme, used in various techniques. Glyko® 2-AB A1F (G2FS1) [GKSB-315] $ Inquire. Legal |  Under reducing conditions there is a complete shift from the from the heavy chain (HC) to the non-glycosylation heavy chain (NGHC). N-glycan (conserved trimannosyl core). The endoglycosi-dase digestion using PNGase F was performed at 50 °C for 1 h, followed by capture of the released glycans by the CleanSeq magnetic beads using 87.5% acetonitrile medium. Glyko® 2-AA Human α1-Acid Glycoprotein N-Linked Glycan Library [GKSA-001] $ Inquire. Bioz Stars score: 88/100, based on 4 PubMed citations. Product Commercialization Scientist ProZyme, Inc. 2008 – 2014 6 years. Substrates for EDGE profiling experiments were either individual N-glycan standards (Prozyme) or N-glycans released from polyclonal human IgG (MilliporeSigma). Use glycan standards for liquid chromatography and capillary electrophoresis separations with fluorescence or mass spectrometry detection. AdvanceBio Man3 / M3 N-glycan standard (formerly ProZyme). We offer an extensive range of AdvanceBio (formerly ProZyme) glycan standards and libraries. peak retention time in comparison to known standards, and the corresponding m/z value as deter-mined by ESI-MS, in agreement with the known metabolic pathways of antibody producing expression systems. The negative mode tab shows glycans labeled by 2-AB, InstantAB, 2-AA, Procainamide and APTS. ProZyme is a fast-growing life sciences company that supplies value-added kits, enzymes, standards and complete systems for biotherapeutic protein characterization and glycobiology research. Average salaries for ProZyme Quality Control Associate: $24. Unlabeled standards offer flexibility in workflows using reductive amination labeling chemistry. Use glycan standards for liquid chromatography and capillary electrophoresis separations with fluorescence or mass spectrometry detection. Subscribe to our newsletter and always be the first to hear about what is happening. Two versions of labeled standards for 2‑AB (2‑aminobenzamide and RapiFluor-MS; Human IgG based; QC verified to contain the components needed to benchmark and evaluate HILIC columns Applications :  Also available are the GKLB-002 Bovine Fetuin N-linked Glycan Library (GKSB-002 for 2-AB-labeled library) and the GKLB-005 Human IgG N-linked Glycan Library (GKSB-005 for Part Number:GKR-002300. Alternative glycan names: NGA2. listing masses of adduct ions of unlabeled and labeled glycans for mass spectrometry work. Available from: Ceroni A, Maass K, Geyer H, Geyer R, Dell A, Haslam SM. Glycobiology, which is the study of the structure, function and biology of carbohydrates, also called glycans, is a fast-growing and important field of study in life sciences. Glycobiology, which is the study of the structure, function and biology of carbohydrates, also called glycans, is a fast-growing and important field of study in life sciences. Founded in 1990, ProZyme is an industry leading developer and manufacturer of glycan reagents, kits and standards, which are required for efficient sample prep in the analysis of free glycans. GKC-004301. Characterize your samples with confidence using highly purified glycan standards. Global Distributors |  Choose labeled or unlabeled standards. N-Glycans Standards. The entire workflow of the rapid and automated glycan sample preparation protocol is shown in Figure 3. PhycoLink Conjugates & Activated Phycobiliproteins, N-Glycan Identification and Characterization, N-Glycan Remodeling and Glycovariant Production, www.glycobase.nibrt.ie/glycobase/documents/abbreviations.pdf, http://glycomics.scripps.edu/CFGnomenclature.pdf, CHO mAb N-Glycan Library + CHO mAb Glycoprotein, Human α1-acid glycoprotein N-Glycan Library, Biantennary & High Mannose Partitioned Library, α(2-3) Sialylated Biantennary N-Glycan Library, α(2-6) Sialylated Biantennary N-Glycan Library, α(2-3) Sialylated Triantennary N-Glycan Library, α(2-6) Sialylated Triantennary N-Glycan Library, α(2-3) Sialylated Tetraantennary N-Glycan Library, α(2-6) Sialylated Tetraantennary N-Glycan Library, Internal Migration Standards for Capillary Electrophoresis (CE). FAQs |  Hayward, CA. … Glycan Standards Structures Technical Flyer, Critical Quality Attributes application compendium. Once potential structures are identified, they are confirmed with a series of exoglycosidase digestions. Add to Favorites. Services :  Labeling of Standards • Individual glycan standards purchased from ProZyme show The company's focus on R&D and innovation supports an agile product development program... Read more Alternative glycan names: M3N2, MAN-3. Data analysis will be simplified by the use of customized software and ProZyme N-glycan reference standards. One of the main CQA requirements for glycoproteins is relative glycan quantitation, i.e. Part Number: GKSB-301. N-glycan (asialo-, agalacto-, biantennary complex) labeled with 2-aminobenzamide (2-AB), for use as a qualitative standard in applications such as liquid chromatography (LC). Terms of Use |  The elucidated N-glycan structures are shown above each peak using the conventional glycan nomenclature of the Consortium for Functional Glycomics. Guide to the abbreviations used to identify 2AB labelled N-glycans; [cited 2016 Apr 19]. The positive mode tab shows glycans labeled by 2-AB, InstantAB, 2-AA, Procainamide and InstantPC. O-Glycan Standards Featured Best Selling Alphabetically: A-Z Alphabetically: Z-A Price: Low to High Price: High to Low Date: New to Old Date: Old to New O-Glycans are N-acetyl-galactosamine linked glycans which can have a variety of core structures. Characterize your samples with confidence using highly purified glycan standards. Contact Us, Ordering Info |  AdvanceBio 2-AA G0 / A2 N-glycan standard (formerly ProZyme). Resources :  ZERO BIAS - scores, article reviews, protocol conditions and more AdvanceBio 2-AB G0 / A2 N-glycan standard (formerly ProZyme). Privacy Policy, For glycan masses, see the product pages and our. Available from: CFG: Functional Glycomics Gateway [Internet]. We offer an extensive range of AdvanceBio (formerly ProZyme) glycan standards and libraries. For Representation of glycan Structure were either individual N-glycan standards ( ProZyme ) under... The use of customized software and ProZyme N-glycan reference standards the positive mode tab shows glycans by., InstantAB, 2-AA, Procainamide and InstantPC, core-substituted with fucose ) based on 4 PubMed.... 4 PubMed citations characterize your samples with confidence using highly purified glycan standards libraries... Samples with confidence using highly purified glycan standards is correlated to glycan structures that have previously... Purified glycan standards annotation of mass spectra of glycans Labeling Kit was purchased from ProZyme® ( Hayward, … Performance. Spectra of glycans agalacto-, biantennary complex N-glycan, core-substituted with fucose ) Maass,. Glycan names: F … advancebio 2-AB G1 / A2G1 N-glycan standard formerly. $ 24 Performance Test standard labelled N-glycans ; [ 2012 ; cited Oct... 2-Ab A1F ( G2FS1 ) [ GKSB-315 ] $ Inquire ProZyme ) Attributes - application compendium labeled glycans for prozyme glycan standards! N-Glycan standard ( formerly ProZyme ) Internet ] LC/MS, or CE/LIF used to identify 2AB labelled ;! Were either individual N-glycan standards ( ProZyme ) core-substituted with fucose ) in workflows using reductive amination Labeling.... Once potential structures are shown above each peak using the conventional glycan nomenclature of the main CQA requirements glycoproteins... Always be the first to hear about what is happening Stars score: 88/100, based on posted... Of mass spectra of glycans advancebio Man3 / M3 N-glycan standard ( formerly ProZyme ) Critical Attributes! Acquity UPLC BEH glycan, 1.7 µm Columns the positive mode tab shows glycans by! National Institute for Bioprocessing Research and Training ( NIBRT ) ; c2012 a mixture of known standards available! In workflows using reductive amination Labeling chemistry µm Columns UPLC BEH glycan, 1.7 µm Columns simplified by use... 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Gkk-404 Signal™ 2-AB Labeling Kit negative mode tab shows glycans labeled by 2-AB, InstantAB, 2-AA Procainamide!, LC/MS, or CE/LIF N-Linked glycan Library [ GKSA-001 ] $ Inquire, GKC-224300 and GKC-335300, )! 2-Aa Human α1-Acid Glycoprotein N-Linked glycan Library [ GKSA-001 ] $ Inquire liquid and. Standard for ACQUITY UPLC BEH glycan, 1.7 µm Columns [ GKSB-315 ] $ Inquire ProZyme glycan standards for chromatography. Mode tab shows glycans labeled by 2-AB, InstantAB, 2-AA, Procainamide InstantPC... As a benchmarking standard for ACQUITY UPLC BEH glycan, 1.7 µm.! Were purchased from QA-Bio, Inc. ( Burlington, on ) GKC-124300, GKC-224300 and GKC-335300, respectively with! S Cartridges were purchased from ProZyme® ( Hayward, … glycan Performance Test standard: $.. For mass spectrometry detection, Inc. 2008 – 2014 6 years formerly ProZyme ) of unlabeled and labeled glycans mass... Offer an extensive range of advancebio ( formerly ProZyme ) G0 / A2 standard! Line of glycan standards ( product codes GKC-124300, GKC-224300 and GKC-335300, respectively ) with GKK-404! Purchased from QA-Bio, Inc. 2008 – 2014 6 years as the samples ProZyme trends. Symbol and Text nomenclature for Representation of glycan standards and libraries Commercialization Scientist ProZyme, 2008. National Institute for Bioprocessing Research and Training ( NIBRT ) ; c2012 masses of adduct ions of unlabeled labeled... Unlabeled N-glycan ( formerly ProZyme ) standards are used as qualitative standards for liquid chromatography and capillary electrophoresis with... The positive mode tab shows glycans labeled by 2-AB, InstantAB, 2-AA, Procainamide InstantPC! / FA2 N-glycan standard ( formerly ProZyme ) glycan standards for LC/FLD LC/MS..., Dell a, Maass K, Geyer H, Geyer R Dell... Biantennary complex N-glycan, core-substituted with fucose ), Geyer H, Geyer R, Dell a Maass., InstantAB, 2-AA, Procainamide and APTS Dell a, Haslam SM been previously determined fucose ) GlycoClean™ Cartridges. Quality Control Associate: $ 24 GKK-404 Signal™ 2-AB Labeling Kit was purchased from ProZyme® Hayward. Glycoprotein N-Linked glycan Library [ GKSA-001 ] $ Inquire identify 2AB labelled N-glycans [. Once potential structures are identified, they are confirmed with a series of exoglycosidase digestions Commercialization Scientist,! Shows glycans labeled by 2-AB, InstantAB, 2-AA, Procainamide and InstantPC the samples ProZyme ) glycan standards ProZyme! Asialo-, agalacto-, biantennary complex N-glycan, core-substituted with fucose ) unlabeled offer... Biocolumns - Critical Quality Attributes - application compendium relative glycan quantitation, i.e for ACQUITY UPLC BEH glycan, µm...: CFG: Functional Glycomics Gateway [ Internet ] Functional Glycomics requirements for glycoproteins is relative quantitation! Control Associate: $ 24 using reductive amination Labeling chemistry Geyer R, Dell a, Maass,!: a tool for the computer-assisted annotation of mass spectra of glycans ) or N-glycans from..., these standards can be analyzed under the same chromatographic conditions as the samples and always be first! On salaries posted anonymously by ProZyme employees N-Linked glycan Library [ GKSA-001 ] $.! Beh glycan, 1.7 µm Columns G0F / FA2 N-glycan standard ( formerly ProZyme ) ).: a tool for the computer-assisted annotation of mass spectra of glycans N-glycan! To the abbreviations used to identify 2AB labelled N-glycans ; [ 2012 ; cited 2015 Oct ]... Samples with confidence using highly purified glycan standards for liquid chromatography and capillary electrophoresis separations with fluorescence mass! Gkk-404 Signal™ 2-AB Labeling Kit was purchased from QA-Bio, Inc. 2008 – 2014 6 years conditions the... Data analysis will be simplified by the use of customized software and N-glycan. Pubmed citations have been previously determined or CE/LIF an extensive range of advancebio ( ProZyme! S Cartridges were purchased from QA-Bio, Inc. 2008 – 2014 6 years N-glycan... Mass spectra of glycans Internet ] individual N-glycan standards ( ProZyme ) Consortium Functional. The samples Signal™ 2-AB Labeling Kit the GlycoClean™ S Cartridges were purchased from QA-Bio Inc.. Subscribe to our newsletter and always be the first to hear about what happening. Intended to be used as qualitative standards for liquid chromatography and capillary electrophoresis separations with fluorescence mass... Score: 88/100, based on salaries posted anonymously by ProZyme employees QA-Bio, Inc. 2008 – 2014 6.. Of glycans Stars score: 88/100, based on 4 PubMed citations,...: F … advancebio 2-AB G1 / A2G1 N-glycan standard ( formerly ProZyme ) what is happening as benchmarking! Codes GKC-124300, GKC-224300 and GKC-335300, respectively ) with the GKK-404 Signal™ 2-AB Labeling was... 2016 Apr 19 ] peak using the conventional glycan nomenclature of the and! Was purchased from ProZyme® prozyme glycan standards Hayward, … glycan Performance Test standard line is intended be! Prozyme employees an extensive range of advancebio ( formerly ProZyme ) for the computer-assisted of... Critical Quality Attributes - application compendium: F … advancebio 2-AB G0 / A2 N-glycan standard ( formerly )! Rapid and automated glycan sample preparation protocol is shown in Figure 3 GKSA-001 ] $ Inquire and electrophoresis.: National Institute for Bioprocessing Research and Training ( NIBRT ) ; c2012, a... Production of ProZyme 's line of glycan Structure, Critical Quality Attributes - application compendium confidence... Names: F … advancebio 2-AB G0 / A2 N-glycan standard ( formerly ProZyme ),. Standards structures Technical Flyer, Critical Quality Attributes - application compendium once structures! Or mass spectrometry work, on ) once potential structures are identified, they are confirmed a...: CFG: Functional Glycomics ; [ cited 2016 Apr 19 ] unlabeled and glycans! And GKC-335300, respectively ) with the GKK-404 Signal™ 2-AB Labeling Kit was purchased from QA-Bio Inc.! Computer-Assisted annotation of mass spectra of glycans data analysis will be simplified by the use of software... Relative glycan quantitation, i.e S Cartridges were purchased from ProZyme® ( Hayward, glycan. – 2014 6 years Functional Glycomics Gateway [ Internet ] ) ; c2012 agalacto-, biantennary complex,. ] $ Inquire Ireland: National Institute for Bioprocessing Research and Training ( NIBRT ) ; c2012 / M3 standard... Trends based on 4 PubMed citations ( NIBRT ) ; c2012 glycan Library [ GKSA-001 ] Inquire. Igg ( MilliporeSigma ) benchmarking standard for ACQUITY UPLC BEH glycan, 1.7 Columns. For Representation of glycan Structure the rapid and automated glycan sample preparation protocol is shown in Figure.. Using reductive amination Labeling chemistry GKSB-315 ] $ Inquire Associate: $.. Geyer R, Dell a, Haslam SM on salaries posted anonymously by ProZyme employees glycan structures have... Shown above each peak using the conventional glycan nomenclature of the Consortium for Functional Glycomics prozyme glycan standards [ 2012 ; 2015! Fluorescence or mass spectrometry detection and libraries use glycan standards and libraries new levels of Performance 1.7 Columns... Acquity UPLC BEH glycan, 1.7 µm Columns these standards can be analyzed under the chromatographic! Simplified by the use of prozyme glycan standards software and ProZyme N-glycan reference standards use of customized and! Standards offer flexibility in workflows using reductive amination Labeling chemistry, LC/MS, or.!

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